Influence of Different Nutrient Sources on Exopolysaccharide Production and Biomass Yield by Submerged Culture of Trametes versicolor and Coprinus sp.

Abstract

Influence of different nutrient sources on Exopolysaccharide (EPS) production and Biomass yield by submerged culture of Trametes versicolor and Coprinus sp. was investigated. The best EPS production by Trametes versicolor and Coprinus sp. was stimulated by sucrose. EPS production ranged within 1,011-17945 mg/l and 200-18,765 mg/l, respectively. The least EPS production was recorded in xylose and glucose, respectively. Xylose and sorbitol supported the highest biomass yield (1.9 gdryw/l and 2.0 gdryw/l) by Trametes versicolor and Coprinus sp., respectively. Yeast extract induced the highest EPS production (7,835 mg/l) by T. versicolor. The EPS ranged within 701-7,835 mg/l. Casein stimulated the highest production of EPS by Coprinus sp. and it ranged within 563-7,474 mg/l. Yeast extract and NaNO3 induced the highest biomass yield by Trametes versicolor and Coprinus sp., respectively. Biomass yield ranged within 1.0-1.6 gdryw/l and 2.0-1.9 gdryw/l. The highest EPS production by the strains was stimulated by glutamic acid and it ranged within 272-3,684 mg/l and 209- 8,899 mg/l, respectively. The least stimulatory amino acids were alanine and glutamate, respectively. The best amino acids for biomass production by the isolates were aspartic acid and asparagines, respectively, and it ranged within 1.0-15.0 gdryw/l and 1.0-16.0 gdryw/l.