Endoglucanase Production by Penicillium atrovenetum Using Plantain Peels as Substrate

Abstract

Production of endoglucanase, a good index for cellulase, by Penicillium atrovenetum using plantain peels as substrate was investigated. Cultural parameters varied include incubation time (1-15 days), pH (3-6), temperature (30-50oC) and nitrogen source. Enzyme production was analyzed by the dinitrosalicyclic acid (DNS) method. Highest production of endoglucanase was observed on the 7th day at pH 5 and at incubation temperature of 40oC. Ammonium oxalate, followed closely by ammonium persulfate, was observed as the best nitrogen source. The crude enzyme has optimum activities at 50oC and pH 5 while stable within 30oC to 50oC and pH 5 to 7. Addition of 5 mM CaCl2 enhanced the activities of the enzyme by 15.54%.